Scientific Studies/Lab Results - Antimicrobial Handwash

Fresh Cleanse anti-bacterial moist towelettes

Antibacterial Lotion

Fresh Cleanse® antimicrobial handwash was developed to fill the need for a non-toxic, non-staining, highly efficacious professional health care handwash that would moisturize and condition skin. This unique antimicrobial handwash complies with FDA testing protocols (21 CFR 333) and qualifies as a Health Care Personnel Handwash. Botanical oils and skin emollients formulated within the Fresh Cleanse® antimicrobial handwash leave skin feeling soft and smooth while it aids in the healing of minor cuts and abrasions.

Independent Scientific Studies and Lab Results

Study 1 - Antimicrobial Efficacy in Vitro:Health Care Personnel Handwash Protocol - Time Kill

A time kill test was performed to determine how rapidly the Fresh Cleanse® antimicrobial handwash kills germs. Test results indicated the handwash removed 99.999% of the bacteria listed below within 15 seconds.


Bacteria	ATCC	Exposure Time	Log10 Reduction	% Reduction
Acinetobacter baumannii	19606	15 Seconds	>5.0128	>99.9990%
Enterobacter cloacae	13047	15 Seconds	>5.2430	>99.9994%
Enterococcus faecalis	29212	15 Seconds	>5.2822	>99.9995%
Enterococcus faecium	19434	15 Seconds	>5.3284	>99.9995%
Escherichia coli	11229	15 Seconds	>5.0645	>99.9991%
Escherichia coli	25922	15 Seconds	>5.4372	>99.9996%
Klebsiella oxytoca	43165	15 Seconds	>5.3493	>99.9996%
Klebsiella pneumoniae	11296	15 Seconds	>5.0846	>99.9992%
Proteus mirabilis	7002	15 Seconds	>5.5378	>99.9997%
Pseudomonas aeruginosa	15442	15 Seconds	>5.1351	>99.9993%
Serratia marcescens	14756	15 Seconds	>5.5185	>99.9997%
Staphylococcus epidermidis	12228	15 Seconds	>5.4843	>99.9997%
Staphylococcus haemolyticus	29970	15 Seconds	>5.0492	>99.9991%
Staphylococcus saprophyticus	15303	15 Seconds	>5.5198	>99.9997%
Streptococcus pneumoniae	6303	15 Seconds	>6.2405	>99.9999%
Candida tropicalis	750	15 Seconds	>5.1038	>99.9992%

Study 2 - Antimicrobial Efficacy in Vivo: Health Care Personnel Handwash Protocol

FDA testing protocols require a 3.0 log reduction by the 10^th wash. The Fresh Cleanse® antimicrobial handwash met the 3.0 log reduction by the 7^th wash.

Antimicrobial Efficacy

Study 3 - Skin Moisture Content

This study measured skin moisture content after repeated use of the Fresh Cleanse® antimicrobial handwash as compared to a baseline (0) and Hibicl+ens 4% CHG handwash product. The Fresh Cleanse® antimicrobial handwash substantially increased skin moisture content and did not cause skin irritation even with repeated use.

Skin Moisture Content

Features	Benefits
FDA compliant (21 CFR 333)	Meets all the FDA testing protocols to qualify as a Health Care Personnel Handwash
CHG/Alcohol/PCMX/Triclosan® free	Non-toxic and fast acting against a broad spectrum of Gram positive and Gram negative bacteria; does not dry out or irritate skin
Proprietary formulation capabilities	First Scientific can develop a proprietary formulation to meet your specific needs
Qualifies as a First-Aid Antiseptic	Does not dry out, sting or irritate skin when used to clean minor cuts and scrapes
Demonstrates persistence	Continues to kill bacteria long after use
Enriched with emollients and natural botanicals that act as skin conditioners	Prevents skin irritation and improves skin condition with repeated use while increasing moisture content; encouraging frequent use
Low sudsing rinses off easily	Quickly administered — saves time
Fresh, clean fragrance	Encourages more frequent handwashing because it is pleasant to use
Latex glove compatible	Does not degrade and allows for quick and easy application of latex gloves

Fresh Cleanse® - (800) 767-2208 - info@freshcleanse.com

Copyright © 1997, First Scientific, Inc. All rights reserved. Fresh Cleanse is a trademark of First Scientific, Inc. Fresh Cleanse products meet or exceed FDA monograph 21 CFR 333 as an antibacterial first-aid solution. Fresh Protect is EPA Registered #683-152.